Acid phosphatases of Ipomoea sp. cultured in vitro. 1. Influence of pH and inorganic phosphate on the formation of phosphatases

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Abstract
The levels and the developmental patterns of the two acid phosphatases in Ipomoea sp. (morning glory) were influenced by the pH of the medium and whether the cultures were grown in fermentors or shake flasks. The two enzymes, which appeared in the culture medium, in the soluble fraction, and in the particulate fraction, were derepressed when suspension cultures were grown in a medium containing low concentrations of inorganic phosphate. The addition of up to 4 μmol of phosphate per millilitre to cells grown for 4 days on low phosphate did not repress the synthesis of the enzymes. However, the addition of excess phosphate resulted in a temporary cessation of phosphatase synthesis. Inorganic phosphate appeared to be only one of several factors controlling the levels of the enzymes.