Isoenzymes of N-Acetyl-β-Glucosaminidase frorn Human Liver and Serum: Separation by Electrofocusing in Thin Layers of Polyacrylamide Gel

Abstract
Isoenzymes of N-acetyl-.beta.-glucosaminidase [EC 3.2.1.30] can be separated by electrofocusing in thin layers of polyacrylamide gel. Patterns of isoenzymes from human liver tissue and serum show changes in liver diseases. Preliminary results indicate that N-acetyl-.beta.-glucosaminidase isoenzyme A is increased in liver tissue from patients with chronic liver diseases in relation to the activity of mesenchyme reaction. The described method will be discussed in comparison with known separation techniques.