Effect of Paclobutrazol on Water Stress-Induced Abscisic Acid in Apple Seedling Leaves
Open Access
- 1 August 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 84 (4) , 1051-1054
- https://doi.org/10.1104/pp.84.4.1051
Abstract
Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) in water-stressed leaves from control apple seedlings, and also from apple seedlings treated for 28 days with paclobutrazol ([2RS, 3RS]-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazol-1-yl] pentan-3-ol). The ELISA quantitative estimates were also validated by gas chromatography-electron capture detector and lettuce seed germination inhibition bioassay. Paclobutrazol treatment reduced endogenous ABA levels by about one-third, and prevented the marked accumulation of water-stress-induced ABA that occurred in untreated seedlings. The presence of ABA in the apple leaf extracts was confirmed by gas chromatography-mass spectrometry.Keywords
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