HEMAGGLUTINATION AND HEMAGGLUTINATION INHIBITION OF TURKEY RED BLOOD-CELLS WITH MYCOPLASMA-HYOPNEUMONIAE
- 1 July 1989
- journal article
- research article
- Vol. 50 (7) , 1052-1055
- https://doi.org/10.2460/ajvr.1989.50.07.1052
Abstract
The ability of Mycoplasma hyopneumoniae to agglutinate RBC was evaluated to develop an in vitro cytadsorption assay. Using swine RBC in a microtitration hemagglutination test, no agglutination or partial agglutination was detected. Comparison of RBC from various other species indicated that improved hemagglutination was obtained with RBC from turkeys. This hemagglutination was detected only when mycoplasma cells used in the assay had been frozen and thawed, heated at 50 C for 30 minutes, or treated with trypsin. Treatment of RBC with trypsin or neuraminidase enhanced hemagglutination. Possible surface lectin activity in M hyopneumoniae was evaluated by use of carboydrates in a blocking assay; hemagglutination was not inhibited by any of 13 carboydrates evaluated. Mycoplasma hyopneumoniae convalescent porcine serum and monoclonal antibodies against 2 M hyopneumoniae immunogens of molecular weights of 64,000 and 41,000 inhibited hemagglutination.This publication has 13 references indexed in Scilit:
- Multiphasic interactions of Mycoplasma pulmonis with erythrocytes defined by adherence and hemagglutinationInfection and Immunity, 1984
- Interaction of Mycoplasma hyopneumoniae with the porcine respiratory epithelium as observed by electron microscopyInfection and Immunity, 1982
- Attachment of Mycoplasma pneumoniae to hamster tracheal organ cultures, tracheal outgrowth monolayers, human erythrocytes, and WiDr human tissue culture cellsInfection and Immunity, 1982
- PREVALENCE OF ANTIBODIES TO HEMOPHILUS-PLEUROPNEUMONIAE IN IOWA SWINE1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Interaction of Mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro modelInfection and Immunity, 1979
- Adherence of Mycoplasma gallisepticum to Human ErythrocytesInfection and Immunity, 1978
- Cytopathogenicity of Mycoplasma hyopneumoniae in porcine tracheal ring and lung explant organ cultures alone and in combination with monolayer cultures of fetal lung fibroblastsInfection and Immunity, 1978
- Platelet plasma membrane lectin activityBiochemical and Biophysical Research Communications, 1977
- SCANNING ELECTRON-MICROSCOPY OF TRACHEA AND BRONCHI FROM GNOTOBIOTIC PIGS INOCULATED WITH MYCOPLASMA-HYOPNEUMONIAE1977