Apoptosis in pressure overload-induced heart hypertrophy in the rat.
Open Access
- 15 June 1996
- journal article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 97 (12) , 2891-2897
- https://doi.org/10.1172/jci118747
Abstract
Pressure overload induces cardiac growth in the rat, which implies the hypertrophy of cardiac muscle cells and proliferation of nonmuscle cells. The cardiac cell loss observed in parallel has generally been attributed to necrosis. Using an in situ assay, we demonstrated a phase of apoptosis or programmed cell death during the first 7 d after pressure overload with a peak at day 4 while cardiac growth continued for over 30 d. The increase in apoptosis was confirmed by quantification of 180-1500-bp DNA oligonucleosomes with agarose gel electrophoresis and in situ labeling via 3'-terminal deoxynucleotidyl transferase assay. While some apoptosis was observed in the basal state in nonmuscle cells, pressure overload induced apoptosis mainly in cardiomyocytes. These data suggest that cardiac hypertrophy is initiated by a wave of apoptosis of cardiomyocytes. Thus, apoptosis may be involved in the pathogenesis of heart remodeling.Keywords
This publication has 26 references indexed in Scilit:
- Apoptosis in Target Organs of HypertensionHypertension, 1995
- Cell Death During Development of the Nervous SystemAnnual Review of Neuroscience, 1991
- Death by suicide: one way to go in mammalian cellular development?1991
- Left ventricular failure induced by long-term hypertension in rats.Circulation Research, 1990
- Nonsynchronous accumulation of alpha-skeletal actin and beta-myosin heavy chain mRNAs during early stages of pressure-overload--induced cardiac hypertrophy demonstrated by in situ hybridization.Circulation Research, 1989
- PATTERNS OF PHYSIOLOGICAL CELL-DEATH AND MITOSES IN THE APICAL ECTODERMAL RIDGE IN NORMODACTYLOUS AND POLYDACTYLOUS RAT LIMB BUDS - A QUANTITATIVE-EVALUATION1989
- Cascade Induction of c-fos, c-myc, and Heat Shock 70K Transcripts during Regression of the Rat Ventral Prostate GlandMolecular Endocrinology, 1988
- Patterns of cell death.1988
- Myocardial reperfusion: a double-edged sword?Journal of Clinical Investigation, 1985
- Normal and hypertrophic growth of the rat heart: changes in cell dimensions and numberAmerican Journal of Physiology-Heart and Circulatory Physiology, 1978