Inhibition by soluble ribonucleic acid of stimulatory effect of liver template ribonucleic acid
- 1 November 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 101 (2) , 448-453
- https://doi.org/10.1042/bj1010448
Abstract
Liver soluble RNA (s-RNA) and, to a smaller extent, Escherichia coli s-RNA inhibited the stimulation of [C14]leucine incorporation into protein in an E. coli S-30 system brought about by liver microsomal RNA or polyuridylic acid as template. The inhibitory activity was associated with the fraction of the S-RNA possessing transfer-RNA activity. The inhibition was exercised at a stage after charging of the s-RNA with amino acid. Neither the method of preparation of the s-RNA nor its state of amino acid acylation affected its inhibitory action. Stimulation of [C14] phenylalanine incorporation by polyuridylic acid or by liver microsomal RNA was not inhibited by addition of s-RNA. It appears that excess of s-RNA inhibits the ambiguous incorporation of leucine with poly-uridylic acid and also that something similar occurs with a natural template. Estimation of messenger activity of samples of RNA should be carried out only after removal of s-RNA.This publication has 7 references indexed in Scilit:
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