ANALYSIS OF THE ANTIGEN-SPECIFICITY OF INFLUENZA HEMAGGLUTININ-IMMUNE HUMAN LYMPHOCYTE-T CLONES - IDENTIFICATION OF AN IMMUNODOMINANT REGION FOR T-CELLS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 50  (4) , 659-666
Abstract
Human T lymphocyte clones specific for the hemagglutinin (HA) molecule of A Texas/1/77 were maintained in long-term culture with T cell-growth factor. The clones were analyzed for their viral antigen specificity using serologically defined type A influenza subtypes and chemically synthesized peptides of the HA-1 molecule. With the exception of 1 T cell clone that recognized only the HA molecule used for immunization, the clones responded to determinants that showed partial or complete cross-reactivity among the strain A subtypes. The cross-reactive population of T cell clones were specific for peptides distinct from the antibody binding sites of HA. One peptide located at the carboxyl terminus of the HA-1 molecule appeared to be immunodominant, although the critical residues for T cell antigen recognition within that could not be identified.

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