PARTIAL-PURIFICATION AND CHARACTERIZATION OF A COLONY-STIMULATING FACTOR SECRETED BY A LYMPHOCYTE-T CLONE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 11  (10) , 931-943
Abstract
The mouse T lymphocyte clone L2 secretes a minimum of 10 lymphokine activities affecting at least 5 different target cells. Large amounts of colony-stimulating factor (CSF) (8.6 .times. 106 U/ml) can be obtained by stimulating L2 cells with concanavalin A. The major CSF activity secreted by L2 cells was enriched to a specific activity of approximately 2-4 .times. 108 colonies/mg of protein using hydrophobic-interaction, gel-permeation, ion-exchange and lectin-affinity chromatography. This preparation of CSF contains no detectable interleukin 2, interleukin 3 or interferon. The major L2-cell CSF induces granulocyte/macrophage colonies from bone marow cells. This GM [granulocyte-macrophage] CSF has an apparent MW of 22,000 as determined by gel-permeation chromatography. Treatment of L2-cell CSF with proteolytic enzymes abrogates biologic activity.

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