A TECHNIQUE FOR OBTAINING THIN SECTIONS OF COCCIDIAN OOCYSTS

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Abstract
A double sectioning technique is described for obtaining thin sections of coccidian [Eimeria brunetti] oocysts. This method employs cryostat sectioning of unfixed oocysts prior to treatment by normal methods of fixation, dehydration, embedding and thin sectioning for EM. A number of oocysts treated by this technique were well preserved and contained organelles with normal ultrastructure.