Aminoacylase pellets
- 1 March 1977
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 19 (3) , 311-321
- https://doi.org/10.1002/bit.260190303
Abstract
Aminoacylase was immobilized on the mycelium pellets of Aspergillus ochraceus by using albumin and glutaraldehyde. No difference in the optimum pH was observed between native aminoacylase and aminoacylase pellets. The amino‐acylase pellets were stable in pH 4–8 but they were unstable in alkaline conditions. The aminoacylase pellets were more stable against heavy metal ions and inhibitors than native aminoacylase. However, the degree of the activation of aminoacylase with cobalt ion decreased with the immobilization. It was suggested that most of aminoacylase was covalently coupled to the mycelium with glutaraldehyde.This publication has 9 references indexed in Scilit:
- Photocontrol of urease-collagen membrane activityBiochimica et Biophysica Acta (BBA) - Enzymology, 1976
- Electric field control of enzyme membrane activityBiochemical and Biophysical Research Communications, 1976
- Immobilized Enzyme TechnologyPublished by Springer Nature ,1975
- New methods for binding enzyme molecules into a water‐insoluble matrix: Properties after insolubilizationBiotechnology & Bioengineering, 1973
- Electrochemical preparation of urease-collagen membraneBiochemical and Biophysical Research Communications, 1972
- Studies on Continuous Enzyme ReactionsAgricultural and Biological Chemistry, 1969
- Studies on continuous enzyme reactions. IV. Preparation of a DEAE‐sephadex–aminoacylase column and continuous optical resolution of acyl‐DL‐amino acidsBiotechnology & Bioengineering, 1967
- Binding of α-amylase to the cell wall of Aspergillus oryzaeBiochimica et Biophysica Acta, 1963
- Studies on Amino Acids. VBulletin of the Agricultural Chemical Society of Japan, 1957