DNA Synthesis and Multinucleation of Mouse Cells Infected with SV40 in the Presence of Cytochalasin B

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Abstract
DNA synthesis and nuclear division of mouse [fibroblast] cells, BALB/3T3, infected with SV-40 were studied and were compared with those of SV-40-transformed mouse cells, mKS-A TU-7. Both SV-40-infected and SV-40-transformed cells behaved similarly in the presence of cytochalasin B and differently from normal non-infected cells, BALB/3T3. The chemical inhibited cytokinesis of all the cell groups but the nuclear division was inhibited only in the case of non-infected BALB/3T3. With SV-40-infected BALB/3T3, multinucleation occurred with the increase of input m.o.i. [multiplicity of infection] by SV-40. SV-40-infected BALB/3T3 could enter the 2nd S phase after release from double thymidine block in the presence of cytochalasin B, while BALB/3T3 could not enter the 2nd S phase. In bi- or multinucleated cells of SV-40-infected BALB/3T3, asynchrony of DNA synthesis among nuclei in a cell was evident, as was the case with mKS-A TU-7.

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