A synthetic peptide containing the autophosphorylation site of the transforming protein of Harvey sarcoma virus is phosphorylated by the EGF‐stimulated tyrosine kinase
Open Access
- 21 March 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 153 (2) , 257-261
- https://doi.org/10.1016/0014-5793(83)80619-x
Abstract
The transforming proteins (p21) of Harvey and Kirsten sarcoma viruses threonine kinase activity, which phosphorylates threonine 59 of the p21 proteins themselves. A tridecapeptide: Arg—Arg—Leu56—Asp—Thr—Thr59—Gly—Gln—Glu—Tyr—Ser—Ala66 containing residues 56‐66 of p21 is phosphorylated solely on tyrosine by the epidermal growth factor (EGF)‐stimulated tyrosine kinase of A431 cell membranes. K m‐Values of 240 and 80 μM and V max values of 1.7 and 0.1 nmol.min−1.mg−1 were obtained in the presence and absence of EGF, respectively.Keywords
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