A 14;18 and an 8;14 chromosome translocation in a cell line derived from an acute B-cell leukemia.

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Abstract
A cell line, named 380, was established from a young male with acute lymphoblastic leukemia (FAB type L2). Karyologic analysis of this cell line indicates that it carries an 8:14 and a 14;18 chromosome translocation, which are characteristic of Burkitt lymphoma and of follicular lymphoma, respectively. This cell line is Epstein-Barr virus antigen-negative, reacts with monoclonal antibodies specific for B cells, and contains rearranged Ig H and L chain genes, but does not express human Ig. In this cell line, both .mu. H chain constant (C.mu.) loci are rearranged within the joining (JH) DNA segment. One of the JH segments on one of the 14q+ chromosomes is rearranged with a segment of chromosome 8, where the c-myc oncogene resides, while the other is rearranged with a segment of chromosome 18 where a putative oncogene, which is called bcl-2, is located. The c-myc oncogene, which is translocated to one of the 14q+ chromosomes, is in its germ-line configuration more than 14 kilobases away from both the JH segment and the H chain enhancer that is located between the JH and .mu. switch region. Based on these findings a model of some aspects of B-cell oncogenesis is proposed according to which B-cell neoplasms carrying translocations involving the H chain loci on both human chromosomes 14 are the result of a multiple step process.