Enzymatic Studies onl-Malic Acid Production fromn-Paraffins byCandida brumptiiIFO-O731

Abstract
Candida brumptii IFO-0731 produced a large amount of l-malic acid from n-paraffins and the acid production was not associated with cell growth. The activities of the enzymes involved in the tricarboxylic acid cycle and the glyoxylate cycle were assayed through cultivation period in order to study on the mechanism ofl-malic acid production from n-paraffins. It was deduced that isocitrate lyase and malate synthase belonging to the glyoxylate cycle played an important role in l-malic acid production.

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