Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor
- 1 January 2002
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 83 (1) , 151-155
- https://doi.org/10.1099/0022-1317-83-1-151
Abstract
A replication-defective form of chimpanzee adenovirus type 68 (C68) has been developed to circumvent problems posed by widespread preexisting immunity to common human adenovirus vectors. To investigate the determinants of C68 tropism, its interaction with the coxsackievirus and adenovirus receptor (CAR) was studied. Although CHO cells were resistant to transduction by C68 as well as by adenovirus type 5 (Ad5), CHO cells expressing either human or murine CAR were transduced readily. C68 transduction, like Ad5 transduction, was blocked when cells were exposed to anti-CAR antibody or when virus was exposed to a soluble form of the CAR extracellular domain. These results indicate that gene delivery by C68 occurs by a CAR-dependent mechanism.Keywords
This publication has 27 references indexed in Scilit:
- Replication-Defective Vector Based on a Chimpanzee AdenovirusJournal of Virology, 2001
- Muscle-Specific Overexpression of the Adenovirus Primary Receptor CAR Overcomes Low Efficiency of Gene Transfer to Mature Skeletal MuscleJournal of Virology, 2001
- Transgenic expression of the coxsackie/adenovirus receptor enables adenoviral-mediated gene delivery in naïve T cellsProceedings of the National Academy of Sciences, 2000
- The Coxsackie-Adenovirus Receptor (CAR) Is Used by Reference Strains and Clinical Isolates Representing All Six Serotypes of Coxsackievirus Group B and by Swine Vesicular Disease VirusVirology, 2000
- Structural Analysis of the Mechanism of Adenovirus Binding to Its Human Cellular Receptor, CARScience, 1999
- Identification of a Conserved Receptor-Binding Site on the Fiber Proteins of CAR-Recognizing AdenoviridaeScience, 1999
- Coxsackie and adenovirus receptor (CAR)-dependent and major histocompatibility complex (MHC) class I-independent uptake of recombinant adenoviruses into human tumour cellsGene Therapy, 1999
- CAR-dependent and CAR-independent pathways of adenovirus vector–mediated gene transfer and expression in human fibroblastsJournal of Clinical Investigation, 1999
- Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5Science, 1997
- Integrins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachmentCell, 1993