Sequential Sampling and Analysis of Renal Hydroxylase Activities of Cattle Given 1α-Hydroxyvitamin D3
Open Access
- 1 April 1986
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 69 (4) , 990-997
- https://doi.org/10.3168/jds.s0022-0302(86)80493-3
Abstract
A new method was developed for sequential sampling of bovine renal cortex. This method results in minimum hemorrhage and adhesions and provides sufficient renal cortex tissue for assay of 25-hydroxyvitamin D 1.alpha., 24-, and 23-hydroxylase activities. Application of this procedure in calves and pregnant cows treated with 1.alpha.-hydroxyvitamin D3 is described. The success of these experiments suggests these techniques could be used to follow enzyme activities that control crucial aspects of vitamin D metbolism in normal peripartum cows and cows with milk fever or other diseases of mineral metabolism.This publication has 19 references indexed in Scilit:
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