MAPK‐dependent expression of p21WAF and p27kip1 in PMA‐induced differentiation of HL60 cells
Open Access
- 20 April 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 472 (1) , 50-52
- https://doi.org/10.1016/s0014-5793(00)01416-2
Abstract
Treatment of HL60 cells with phorbol 12‐myristate 13‐acetate (PMA) results in growth arrest and differentiation towards the macrophage lineage. PMA‐induced changes are easily monitored by morphological changes while cells in suspension start adhering onto substrate. PMA induces rapid activation of the extracellular signal‐regulated kinases (ERKs). Activation of the ERK pathway is essential to PMA‐induced differentiation of HL60 cells. PMA also induces the expression of the cyclin‐dependent kinase inhibitors p21WAF and p27kip1, which is modulated by the use of an inhibitor of the ERK cascade. This implies that a link exists between ERK activation and p21WAF and p27kip1 induction in the process of terminal differentiation.Keywords
This publication has 10 references indexed in Scilit:
- Sustained Activation of the Extracellular Signal-regulated Kinase/Mitogen-activated Protein Kinase Pathway Is Required for Megakaryocytic Differentiation of K562 CellsPublished by Elsevier ,1997
- A Novel, Nerve Growth Factor-activated Pathway Involving Nitric Oxide, p53, and p21WAF1 Regulates Neuronal Differentiation of PC12 CellsPublished by Elsevier ,1997
- Cell cycle arrest mediated by the MEK/mitogen-activated protein kinase pathwayProceedings of the National Academy of Sciences, 1997
- Inhibitors of Cyclin-dependent Kinases Promote Survival of Post-mitotic Neuronally Differentiated PC12 Cells and Sympathetic NeuronsJournal of Biological Chemistry, 1996
- p53-Independent Expression of p21 Cip1 in Muscle and Other Terminally Differentiating CellsScience, 1995
- WAF1, a potential mediator of p53 tumor suppressionCell, 1993
- The beta-PDGF receptor induces neuronal differentiation of PC12 cells.Molecular Biology of the Cell, 1992
- Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.Journal of Biological Chemistry, 1990
- Nerve growth factor stimulates a protein kinase in PC-12 cells that phosphorylates microtubule-associated protein-2.Journal of Biological Chemistry, 1990
- Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase.Proceedings of the National Academy of Sciences, 1989