Quantitative Bausteinanalyse der Stützmembran in der Zellwand von Escherichia coli B

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Abstract
The three concentric layers of the cell wall of Escherichia coli B (the outer lipo-protein layer, the intermediate lipopolysaccharide layer and the inner rigid layer containing protein and mucopolymer) contribute 60%, 12% and 21%, to the total weight of the cell wall. Treatment of the rigid layer with proteolytic enzymes removes the bulk of its protein components: still rigid and cell-shaped it is found to contain glucose, lipid and the mucopeptide constituents muramic acid, glucosamine, diaminopimelic acid, glutamic acid and alanine in molar ratios corresponding to the chemical compositions of known enzymatic mucopolymer fission products from E. coli walls.

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