Inhibition of Growth of Chlorella Pyrenoidosa by Beta-emitting Radioisotopes.

Abstract
Chlorella pyrenoidosa cells were grown for 3 days in the presence of several concns. of H23O, P32, S35 and Sr90-Y90 and then subcultured for 3 days in nonradioactive inorganic nutrient solution. Growth rates of cells were decreased in proportion to the dose of radiation received above approx. 1000 rep/day. The reduced rate of growth continued in subculture with time (for the 3-day period) as a function of the dose received. An increase in the average cell size also occurred during the growth of the algae in the radioactive solns. The results reported do not allow a deduction of the specific site of action of the ionizing radiations in the algae. However, several possible modes of action consistent with the reported results are discussed.

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