Inhibition of Hog Liver Crystalline Quinolinate Phosphoribosyltransferase by Nucleotides, Quinolinate Analogues and Sulfhydryl Reagents
- 1 February 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 40 (2) , 385-389
- https://doi.org/10.1080/00021369.1976.10862052
Abstract
Effects of the precursors and intermediates of the NAD biosynthetic pathway, and of quinolinate analogues etc. on hog liver crystalline quinolinate phosphoribosyltransferase (an intermediary enzyme in the de novo NAD biosynthetic pathway) activity were investigated. The enzyme activity was inhibited by many kinds of nucleotides, phthalic acid and SH reagents. But amino acids, nicotinic acid and nicotinamide had practically no effect. The apparent inhibition by ATP removed by raising Mg2+ concentration. Phthalic acid was proved to be a competitive inhibitor to quinolinic acid. The Ki value for phthalic acid was calculated at 1.7 × 10−4 m by a Dixon plot.This publication has 3 references indexed in Scilit:
- The Enzymatic Conversion of Quinolinate to Nicotinic Acid Mononucleotide in Mammalian LiverPublished by Elsevier ,2021
- The effect of the oral administration of leucine on the metabolism of tryptophanBiochemical Journal, 1963
- The determination of enzyme inhibitor constantsBiochemical Journal, 1953