Factors affecting the hydrogen cyanide potential of white clover (Trifolium repens L.)

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Abstract
Pot experiments were used to determine the effects of age, light, temperature, mineral nutrition and wilting on the HCN potential (HCNp ) of white clover (Trifolium repens L.). HCNp, adjusted for pre-expenmental HCNp, was reduced by higher light intensity (505 v. 220 mg HCN kg-1 DM), by increased temperature (408 v. 317 mg HCN kg-1 DM) and by phosphorus application (382 v. 343 mg HCN kg-1 DM). These effects were largely explained by the hypothesis that HCNp diminished as the size and vigour of the plant increased, and the use of dry matter production as a covariate on HCNp removed the significant effects of light, temperature and phosphorus. In two experiments wilting plants prior to harvest increased HCNp by 15 and 24%, and reduced dry matter yield by 9 and 13%. HCNp declined curvilinearly as the white clover aged. The conditions shown to favour high HCNp, namely, immaturity, moisture stress, low light intensity, cool temperature, and inadequate P supply, are those which occur periodically in the late autumn and early spring of temperate climates. Sheep in late pregnancy at this time, could, if white clover comprises a substantial proportion of their diet, experience thiocyanate absorption as a consequence of HCN detoxication and hence increase the risk of goitri to the lamb.