DNA sequences required for expression of a Dictyostelium actin gene.

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Abstract
A 2.8‐kb fragment of 5′ non‐coding DNA from the Dictyostelium actin 15 gene has previously been shown to contain all of the cis‐acting DNA sequence elements required for normal developmentally‐regulated transcription of actin gene fusion RNAs when reintroduced into the genome by DNA‐mediated transformation. Deletion analysis of this promoter fragment indicates that all of the necessary information is contained within a 270‐bp fragment of actin 15 5′ non‐coding DNA. This fragment contains four short G/C‐rich repeated sequences that are also found in other co‐regulated Dictyostelium actin genes. A 12‐bp consensus sequence, AAAAATGGGG/ATT, is present in the regions essential for expression of two different Dictyostelium actin genes, actin 6 and actin 15, but is absent from an actin gene showing a different temporal pattern of developmental regulation. Deletion analysis and DNase I footprinting implicate this sequence as a functional cis‐acting element required for transcription of the actin 15 gene.