A HAEMOLYTIC SPOT TEST FOR THE ESTIMATION OF ALLOANTIBODY AGAINST ANTIGENS OF THE MAJOR HISTOCOMPATIBILITY COMPLEX
- 1 November 1976
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 22 (5) , 415-419
- https://doi.org/10.1097/00007890-197611000-00001
Abstract
A haemolytic technique for the assay of alloantibody has been developed. Titration of alloantiserum was accomplished by placing microdroplets of serum dilution on agarose layers containing the appropriate erythrocytes. Complement-mediated lysis was procured after the addition of rabbit serum. The assay appeared specific for products of the major histocompatibility gene complex in the rat and in the mouse.Keywords
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