The Structure of Lipid A of the Lipopolysaccharide from Burkholderia caryophylli with a 4‐Amino‐4‐deoxy‐L‐arabinopyranose 1‐Phosphate Residue Exclusively in Glycosidic Linkage
- 20 March 2003
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 9 (7) , 1542-1548
- https://doi.org/10.1002/chem.200390177
Abstract
From the lipopolysaccharides (LPSs) of the plant‐pathogenic bacterium Burkholderia caryophylli, the complete structure of lipid A has been characterized. For the first time, a 4‐amino‐4‐deoxy‐L‐arabinopyranose 1‐phosphate residue was proven to be exclusively linked to the reducing end of lipid A from a wild‐type LPS. The LPSs of B. caryophylli were degraded by mild acetate buffer hydrolysis at pH 4.4. The obtained lipid A was analyzed as such, and also after de‐O‐acylation or dephosphorylation. The structure of lipid A was identified mainly by means of matrix‐assisted laser desorption/ionisation mass spectrometry, and by various 1D and 2D 1H and 13C NMR spectroscopic measurements.Keywords
This publication has 25 references indexed in Scilit:
- Accumulation of a Polyisoprene-linked Amino Sugar in Polymyxin-resistant Salmonella typhimurium andEscherichia coliJournal of Biological Chemistry, 2001
- An Inner Membrane Enzyme in Salmonellaand Escherichia coli That Transfers 4-Amino-4-deoxy-l-arabinose to Lipid AJournal of Biological Chemistry, 2001
- Invited review: Lipopolysaccharide biosynthesis: which steps do bacteria need to survive?Innate Immunity, 2001
- High-resolution NMR Spectroscopy of Lipid A Molecules Containing 4-Amino-4-deoxy-l-arabinose and Phosphoethanolamine SubstituentsJournal of Biological Chemistry, 2000
- Resistance of Serratia marcescens to polymyxin B: a comparative investigation of two S-form lipopolysaccharides obtained from a sensitive and a resistant variant of strain 111Innate Immunity, 1996
- Characterization of Lipopolysaccharides of Polymyxin‐Resistant and Polymyxin‐Sensitive Klebsiella pneumoniae O3European Journal of Biochemistry, 1996
- An NMR Spectroscopy and Molecular Mechanics Study of the Molecular Basis for the Supramolecular Structure of LipopolysaccharidesBiochemistry, 1996
- Analysis of lipopolysaccharide (lipid A) fatty acidsJournal of Microbiological Methods, 1990
- Characterization of the lipopolysaccharide from the polymyxin‐resistant pmrA mutants of Salmonella typhimuriumFEBS Letters, 1981
- Absolute Configuration of 3-Hydroxy Fatty Acids Present in Lipopolysaccharides from Various Bacterial GroupsEuropean Journal of Biochemistry, 1976