Isolation of human rotavirus subgroups 1 and 2 in cell culture
- 1 October 1982
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 16 (4) , 727-730
- https://doi.org/10.1128/jcm.16.4.727-730.1982
Abstract
One strain of human rotavirus subgroup 1 (KUN) and 1 strain of subgroup 2 (MO) were isolated with the MA104 cell line, a fetal rhesus monkey kidney cell line. Their subgroup specificities and RNA patterns were identical to those of rotaviruses present in stools before cultivation. Distinct cytopathic effects consisting of obscure cell boundaries, cell fusion, cell rounding, cell detachment and lytic foci were recognized at passage 3 of MO and passage 6 of KUN. No differences in cytopathic changes were found between the 2 isolates.This publication has 13 references indexed in Scilit:
- Definition of human rotavirus serotypes by plaque reduction assayInfection and Immunity, 1982
- Two Distinct Electrophoretic Migration Patterns of RNA Segments of Human Rotaviruses Prevalent in Japan in Relation to Their SerotypesMicrobiology and Immunology, 1982
- Sequential Passages of Human Rotavirus in MA‐104 CellsMicrobiology and Immunology, 1981
- Isolation of human rotavirus in cell culturesArchiv für die gesamte Virusforschung, 1981
- Comparison of human rotaviruses isolated in Mexico City and in Santiago, Chile, by electrophoretic migration of their double-stranded ribonucleic acid genome segmentsInfection and Immunity, 1980
- Human Rotavirus Type 2: Cultivation in VitroScience, 1980
- Viral gastroenteritis.1979
- Reovirus-Like Agent in Acute Epidemic Gastroenteritis in Japanese Infants: Fecal Shedding and Serologic ResponseThe Journal of Infectious Diseases, 1977
- Recent Advances in the Aetiology of Viral GastroenteritisPublished by Wiley ,1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970