Evidence That More Than One Gene Encodes n-Alkane-inducible Cytochrome P-450s in Candida maltosa, Found by Two-Step Gene Disruption.
- 1 January 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 55 (7) , 1757-1764
- https://doi.org/10.1271/bbb1961.55.1757
Abstract
An n-alkane-assimilating yeast, Candida maltosa, has a diploid genome. Probed with the previously isolated gene of n-alkane-inducible cytochrome P-450 (P-450alk), its allelic gene had been isolated, its nucleotides sequenced, and the interallelic divergence examined. Using one of the allelic genes, we disrupted the two alleles of the cytochrome P-450alk gene by a two-step gene disruption system. Surprisingly, the disruptant still assimilated n-alkane and contained n-alkane-inducible cytochrome P-450. This result indicates that, other than the disrupted two alleles, there is at least one other gene that encodes an n-alkane-inducible cytochrome P-450.Keywords
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