Mode of action of bacteriophage &phi149 on cholera and E1 Tor vibrios
- 1 December 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 24 (12) , 1583-1589
- https://doi.org/10.1139/m78-253
Abstract
Bacteriophage .vphi.149 propagated in Vibrio cholerae (classical) OGAWA 154 strain killed V. cholerae (El Tor) strain MAK 757 without phage propagation. El Tor vibrios underwent a small degree of lysis only when infected by the phage .vphi.149 at a high multiplicity of infection and lost their viability at a rate-dependent multiplicity of phage infection. Evidence was obtained with 32P-labeled bacteriophage .vphi.149 for penetration of phage DNA into both bacterial strains. In host strain (OGAWA 154) phage particle synthesis occurred normally. In El Tor strain MAK 757 the phage DNA was not degraded but its expression was blocked. The killing effect of .vphi.149 on El Tor strain MAK 757 is supposed to be due to damage of the cytoplasmic membrane, which could not be repaired under the influence of phage information. This was indicated by a blockage of cellular respiration and RNA and protein synthesis.Keywords
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