Genomic Analysis of Different Chlorobium Strains by Pulsed-Field Gel Electrophoresis and Ribotyping
- 1 October 1996
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 46 (4) , 1177-1179
- https://doi.org/10.1099/00207713-46-4-1177
Abstract
Pulsed-field gel electrophoresis was used to characterize the genomes of 15 Chlorobium strains. An analysis of chromosomal macrorestriction patterns allowed us to determine the chromosome sizes, which ranged from 1,435 to 3,342 kb depending on the strain. Moreover, this analysis revealed that, even though the genus Chlorobium is a phylogenetically coherent taxon, there is a great deal of genomic heterogeneity within it. This heterogeneity was corroborated by performing a ribotyping analysis, which showed that the rRNA hybridization patterns of most strains are unique.Keywords
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