The structure of the carbohydrate backbone of the core‐lipid A region of the lipopolysaccharide from a clinical isolate of Yersinia enterocolitica O:9

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Abstract
Yersinia enterocolitica O:9 strain Ruokola/71‐c‐ΦR1‐37‐R possesses mainly rough‐type lipopolysaccaride (LPS) and smaller amounts of S‐form LPS. Structural analysis of the former is reported here. After deacylation of the LPS, the phosphorylated carbohydrate backbone of the inner core‐lipid A region could be isolated by using high‐performance anion‐exchange chromatography. Its structure was determined by means of compositional and methylation analyses and 1H‐, 13C‐, and 31P‐NMR spectroscopy as:in which l‐α‐d‐Hep is lglycero‐α‐dmanno‐heptopyranose, d‐α‐d‐Hep is dglycero‐α‐dmanno‐heptopyranose, and Kdo is 3‐deoxy‐dmanno‐oct‐2‐ulopyranosonic acid. All hexoses are pyranoses.