Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli
- 1 June 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 203 (3) , 769-773
- https://doi.org/10.1042/bj2030769
Abstract
1. Neurospora crassa catabolic dehydroquinase has been purified from N. crassa and Escherichia coli. 2. Protein-sequence and gel-electrophoretic data show that apparently pure, homogeneous native dehydroquinase is a mixture of intact and proteinase-cleaved enzyme monomers. 3. Protein-sequence data and steady-state kinetics show that the catabolic dehydroquinase gene of N. crassa is expressed with fidelity in E. coli.This publication has 29 references indexed in Scilit:
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