Tissue culture, protein and collagen synthesis in antibiotic sterilized canine heart valves
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Cardiovascular Research
- Vol. 10 (3) , 389-393
- https://doi.org/10.1093/cvr/10.3.389
Abstract
Viability of canine heart valve leaflet fibroblasts was assessed after varying periods of sterilization and storage in antibiotic-nutrient solution. Tissue culture and assessment of protein and collagen synthesis showed that tissue obtained undsr optimal conditions rarely retains viability beyond 3 weeks in antibiotic-nutrient solution and is severely impaired after 2 weeks. This casts serious doubts on viability in current clinical homograft valve practice.This publication has 2 references indexed in Scilit:
- The behaviour of homotransplanted aortic valves in the dogBritish Journal of Surgery, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951