Use of Derivative Spectrophotometry in the Resolution of Overlapped Peaks in Liquid Chromatography and Its Application in the Analysis of Active Components in Insecticide Formulations

Abstract
A procedure for the determination of active components in insecticide formulations showing overlapped peaks in liquid chromatography has been devised. The procedure is based in the use of derivative spectra of the components obtained by a diode-array spectrophotometer around the maxima signal of the chromatographic peak, and has been applied to the analysis of mixtures of piperonyl butoxide, neopynamine and fenitrothion with satisfactory results.