Structural plasticity broadens the specificity of an engineered protease
- 1 May 1989
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 339 (6221) , 191-195
- https://doi.org/10.1038/339191a0
Abstract
The substrate specificity of α-lytic protease has been changed dramatically, with a concomitant increase in activity, by replacing an active-site Met with Ala. The substrate specificity of both this mutant and another similar mutant are extraordinarily broad. X-ray crystallographic analysis shows that structural plasticity, a combination of alternate side-chain conformations and binding-site flexibility, allows both large and small substrates to be well accommodated.Keywords
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