The proteasome 11S regulator subunit REGα (PA28α) is a heptamer
Open Access
- 1 November 1997
- journal article
- for the-record
- Published by Wiley in Protein Science
- Vol. 6 (11) , 2469-2473
- https://doi.org/10.1002/pro.5560061123
Abstract
Activity of the 20s proteasome, which performs much of the cytosolic and nuclear proteolysis in eukaryotic cells, is controlled by regulatory complexes that bind to one or both ends of the cylindrical proteasome. One of these complexes, the 11S regulator (REG), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. REG, purified from red blood cells, is a complex of REGα and REGβ subunits. We have crystallized recombinant REGα (rREGα) and collected diffraction data to 3.0 Å resolution. The self-rotation function indicates that rREGα forms a heptameric ring in the crystal. Equilibrium sedimentation demonstrates that rREGα is a heptamer in solution also.Keywords
This publication has 37 references indexed in Scilit:
- Solvent content of protein crystalsPublished by Elsevier ,2006
- Structure of 20S proteasome from yeast at 2.4Å resolutionNature, 1997
- A Model for the Quaternary Structure of the Proteasome Activator PA28Journal of Biological Chemistry, 1996
- Primary structures of two homologous subunits of PA28, a γ‐interferon‐inducible protein activator of the 20S proteasomeFEBS Letters, 1995
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994
- KEKE motifsFEBS Letters, 1994
- Evidence That the Nature of Amino Acid Residues in the P3 Position Directs Substrates to Distinct Catalytic Sites of the Pituitary Multicatalytic Proteinase Complex (Proteasome)Biochemistry, 1994
- PA28 Activator Protein Forms Regulatory Caps on Proteasome Stacked RingsJournal of Molecular Biology, 1994
- Structural Features of the 26 S Proteasome ComplexJournal of Molecular Biology, 1993
- Proteasome and its novel endogeneous activator in human plateletsBiochemical and Biophysical Research Communications, 1991