Evidence that KpsT, the ATP-binding component of an ATP-binding cassette transporter, is exposed to the periplasm and associates with polymer during translocation of the polysialic acid capsule of Escherichia coli K1
Open Access
- 1 February 1997
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 179 (4) , 1400-1403
- https://doi.org/10.1128/jb.179.4.1400-1403.1997
Abstract
KpsT utilizes ATP to effect translocation of the polysialic acid capsule of Escherichia coli K1. We have previously proposed a mechanistic model for the action of this protein. Here, we provide evidence to support two predictions of the model: that KpsT associates with polymer and that KpsT is accessible from the periplasmic surface of the inner membrane.Keywords
This publication has 25 references indexed in Scilit:
- Coating the surface: a model for expression of capsular polysialic acid in Escherichia coli K1Molecular Microbiology, 1996
- Polysialic acid export in Escherichia coli K1: the role of KpsT, the ATP-binding component of an ABC transporter, in chain translocationGlycobiology, 1996
- SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecFCell, 1995
- Bacterial polysaccharides in sickness and in healthMicrobiology, 1995
- SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active stateCell, 1994
- SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertionCell, 1994
- Two distinct ATP‐binding domains are needed to promote protein export by Escherichia coli SecA ATPaseMolecular Microbiology, 1993
- ABC Transporters: From Microorganisms to ManAnnual Review of Cell Biology, 1992
- Traffic ATPases: A Superfamily of Transport Proteins Operating from Escherichia coli to HumansPublished by Wiley ,1992
- Molecular analysis of the Escherichia coli K5 kps locus: identification and characterization of an inner‐membrane capsular polysaccharide transport systemMolecular Microbiology, 1990