Abstract
Mycelium of Neocosmospora vasinfecta E. F. Smith grown in media of restricted Mg2+ supply was used for experiments on Mg2+ uptake. The mycelium carried out adsorption and influx of Mg2+. Two binding species at the mycelial surface were responsible for Mg2+ adsorption, with association constants of 2.1 × 106 and 1.6 × 104 L/mol, respectively. Influx of Mg2+ within the range of 5 to 500 μM Mg2+ took place via a single kinetically distinct transport system with an apparent Km for Mg2+ of 30 to 40 μM. Influx of Mg2+ varied markedly with mycelial Mg2+ status, whereas efflux appeared much less variable in rate. The results indicated that internal mycelial Mg2+ content is regulated by modulation of Mg2+ influx.
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