O6-Methylguanine-DNA transmethylase converts O6-methylguanine thymine base pairs to guanine thymine base pairs in DNA

Abstract
O 6 -Methylguanine lesions in natural and synthetic DNAs were studied as substrates for the O 6 -methylguanine-DNA methyltransferase in vitro . The results indicate that O 6 -methylguanine is repaired by this protein when base paired to T as well as to C in double-stranded DNA. The results also indicate that O 6 -methylguanine is less subject to repair than previously reported when it is in single-stranded. DNA and suggest that O 6 -methylguanine may not be repaired when it is at the 3′ terminus.

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