Repeated tuberculin testing does not induce false positive ELISPOT results

Abstract
ESAT-6 and CFP-10 are, however, contained within tuberculin purified protein derivative (PPD). Since ELISPOT is a highly sensitive method for measuring even low numbers of antigen specific T cells,4 concerns have been raised as to whether repeated tuberculin skin tests might induce T cell responses to these specific antigens, resulting in false positive ELISPOT results.