A new approach for using cofactor dependent enzymes: example of alcohol dehydrogenase
- 29 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (2) , 321-324
- https://doi.org/10.1016/0014-5793(84)81188-6
Abstract
The use of enzymes requiring a cofactor as substrate in organic synthesis is still a problem since the cofactors are expensive. This study deals with a new approach consisting of using fragments of NAD+. Three fragments of NAD(H) are examined. The activities of NMN+ and NMNH are greatly improved by the addition of adenosine in ethanol oxidation and in cyclohexanone reduction, respectively. Nicotinamide monocucleoside is not active in the ethanol oxidation but the addition of AMP promotes this reaction.Keywords
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