The General Properties of a Strain of Yeast with Less DNA than Normal

Publisher Website
Google Scholar
Abstract
SUMMARY A strain of yeast called 2n~ is described which behaved genetically as a diploid, but had approximately 18 % less DNA than a normal diploid. It produced apparently normal haploid ascospore cultures, which had approximately 20 yo less DNA than normal haploids. The growth of 2n~ from low yeast concentrations was normal, but at high concentrations it was abnormal; viability was not affected. The timing of DNA synthesis in a synchronously dividing culture was in the usual position in the growth cycle for yeast. DNA was estimated by the diphenyl- arnine reaction; there was no special effect on this reaction, peculiar to 2n~ and its ascospore cultures. DNA values were confirmed by Azao measurements. The yeast 2n~ was more sensitive to both gamma and U.V. radiations than were normal diploids and there was some indication that defective repair processes were involved.

This publication has 0 references indexed in Scilit: