Co-ordination of nickel(II) ions by angiotensin II and its peptide fragments. A potentiometric, proton nuclear magnetic resonance and circular dichroism spectroscopic study
- 1 January 1989
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in J. Chem. Soc., Dalton Trans.
- No. 8,p. 1471-1475
- https://doi.org/10.1039/dt9890001471
Abstract
A potentiometric and spectroscopic (n.m.r., circular dichroism, and u.v.–visible) study of the complexes of angiotensin II and two of its peptide fragments, Asp-Arg-Val-Tyr and MeCO-Tyr-IIe-His, with NiII has been undertaken. The results show that, above pH 8, NiII co-ordinates to four nitrogen donors starting at the terminal amino nitrogen of the aspartyl residue to form a diamagnetic, planar complex. Co-ordination through the imidazole N of the histidyl residue may be present below pH 8–9 but is absent at high pH. The high-resolution 1H n.m.r. spectrum of angiotensin II and of its nickel(II) complex at pH 11 have been recorded and the side-chain resonances assigned.This publication has 1 reference indexed in Scilit:
- Ca2 + and Mg 2+ dependence of angiotensin II binding to isolated rat renal glomeruliBiochemical Pharmacology, 1978