Using Biofunctional Magnetic Nanoparticles to Capture Vancomycin-Resistant Enterococci and Other Gram-Positive Bacteria at Ultralow Concentration
Top Cited Papers
- 26 November 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (51) , 15702-15703
- https://doi.org/10.1021/ja0359310
Abstract
Covalently linked to vancomycin (Van), chemically stable and highly magnetic anisotropic FePt magnetic nanoparticles (3−4 nm) become water-soluble and capture vancomycin-resistant enterococci (VRE) and other Gram-positive bacteria at concentrations ∼101 cfu/mL via polyvalent ligand−receptor interactions. When a pyramidal end of a magnet “focuses” the nanoparticles into ∼1 mm2 area, the bacteria can be observed by an optical microscope and further identified by electron micrograph (EM). Compared to the conventional use of magnetic particles (with the sizes of 1−5 μm) in biological separation or drug delivery, magnetic nanoparticles, combined with specific receptor−ligand interactions, promise a sensitive and rapid protocol to detect pathogens.Keywords
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