CONTINUOUS DETECTION OF ACETYLCHOLINE-RELEASE IN ELECTRIC ORGAN OF TORPEDO BY A CHEMI-LUMINESCENT PROCEDURE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 291  (8) , 713-716
Abstract
A chemiluminescent procedure to measure acetylcholine was developed and used to detect continuously the release of transmitter from stimulated Torpedo electric organ slices and synaptosomes. Acetylcholine is hydrolyzed by acetylcholinesterase; the choline liberated is oxidized by choline oxidase to betaine; the H2O2 generated triggers the luminescence of luminol in presence of peroxidase. The extension to other transmitters is possible and several developments are envisaged.

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