Characteristics of the induction of phosphodiesterases by cyclic adenosine 3',5'-monophosphate in the slime mold, Dictyostelium discoideum.

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Abstract
Cyclic AMP (cAMP) stimulated the induction of cAMP-phosphodiesterases of the slime mold, D. discoideum. The membrane phosphodiesterase was induced by more than 10-5 M of cAMP concentration, while the extracellular enzyme was done 3.5 times higher by 10-6 M of cAMP compared with free cAMP. The induction of the extracellular phosphodiesterase by cAMP was inhibited by EDTA, p-chloromercuribenzene sulfonate (PCMB), concanavalin A (Con A) and progesterone all of which are known to cease differentiation of the slime mold. The derivation of the extracellular enzyme was also stopped by dithiothreitol which reversely accelerates development of the cells. Con A and dithiothreitol further stimulated the induction of the membrane phosphodiesterase by cAMP more than by cyclic nucleotide alone. cGMP and N6,O2''-dibutyryl cAMP are known to have less chemotaxis-characteristics for the slime mold than cAMP. These cAMP-analogs occurred in the synthesis of the membrane and the extracellular phosphodiesterases to the same or greater extent. When the surface of the slime mold was treated with trypsin, the induction of extracellular phosphodiesterase by cAMP was suppressed to half. Apparently the induction of synthesis of phosphodiesterases by cAMP in the slime mold is independent of the occurrence of chemotaxis.

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