Properties of the Reverse Transcription Reaction in mRNA Quantification
Open Access
- 1 March 2004
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 50 (3) , 509-515
- https://doi.org/10.1373/clinchem.2003.026161
Abstract
Background: In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression.Keywords
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