Detection of antibodies against avian leukosis viruses with indirect immunoperoxidase absorbance test.

Abstract
The indirect immunoperoxidase absorbance (IIPA) test was used to detect chicken serum antibody against avian leukosis viruses (ALV). Total 155 serum samples collected from chickens experimentally infected and not infected with ALV were tested by the method and the result was compared with that of the virus neutralization (VN) test. As a result, there was a high correlation (R = 0.648) between the 2. The sera of chickens infected with Rous-associated virus (RAV) showed type-specificity when examined by the method. Thus, those infected with RAV-1 were clearly distinguished from those infected with RAV-2. Eight chickens were then inoculated with RAV-1 to study the rise and fall in antibodies. IIPA antibody presented almost the same rise and fall as VN antibody in these chickens.