Tandemly repeated nonribosomal DNA sequences in the chloroplast genome of an Acetabularia mediterranea strain.

Publisher Website
Google Scholar
Abstract
A purified chloroplast fraction was prepared from caps of the giant unicellular green alga A. mediterranea (strain 17). High MW DNA obtained from these chloroplasts contains at least 5 copies of a 10-kilobase-pair (kbp) sequence tandemly arranged. This unique sequence is present in DNA from chloroplasts of all stages of the life cycle examined. A chloroplast rDNA clone from mustard hybridized with some restriction fragments from Acetabularia chloroplast DNA but not with the repeated sequence. An 8-kbp EcoRI-Pst I fragment of the repeated sequence was cloned into pBR322 and used as a hybridization probe. No homology was found between the cloned 8-kbp sequence and chloroplast DNA from related species A. crenulata or chloroplast DNA from spinach.