Characterization of chromatin modified with ethyl acetimidate
- 23 August 1977
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 16 (17) , 3746-3753
- https://doi.org/10.1021/bi00636a003
Abstract
Thymus [calf, rabbit] chromatin was extensively modified with ethyl actimidate, substituting up to 90% of the lysyl residues of the histones while retaining the positive charge of the basic amino acid. Physicochemical and immunochemical characterization of this derivative chromatin indicates a high degree of retention of the native structure of the nucleoprotein even after extensive modification. The alterations which are detected are most simply interpreted as resulting from a weakening of the interactions of histone H1 with DNA in the modified chromatin. The near-native character of amidinated chromatin contrasts with the more extensive structural alterations observed in acetylated chromatin. The data demonstrate the suitability of this reagent for mapping available lysyl residues in this and other nucleoproteins and suggest that the related bisimido esters may be reagents of choice for cross-linking of chromatin histones.This publication has 9 references indexed in Scilit:
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