High-performance liquid chromatographic separation of citraconylinsulins and preparation of GlyA1PheB1-dicitraconylinsulin.
- 1 January 1985
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 33 (3) , 1159-1163
- https://doi.org/10.1248/cpb.33.1159
Abstract
A method for the mutual separation of 6 citraconylated porcine insulins and intact insulin from the reaction mixture of insulin with citraconic anhydride is described, based upon anion-exchange high-performance liquid chromatography on a TSK IEX-540 DEAE column. The method permits the preparative isolation of citraconylinsulins. The optimal reaction conditions for the preparation of GlyA1Phe B1-dicitraconylinsulin, a useful starting material for the preparation of Lys-B29 modified insulin derivatives, were also investigated.This publication has 1 reference indexed in Scilit:
- [49] Reaction of proteins with citraconic anhydridePublished by Elsevier ,1972