Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases
- 1 June 1999
- journal article
- review article
- Published by Springer Nature in Nature Cell Biology
- Vol. 1 (2) , E25-E27
- https://doi.org/10.1038/10018
Abstract
One of the most powerful ways of studying the function of a protein is to specifically block its activity within cells. Over the past decade, dominant-inhibitory proteins have emerged as popular tools with which to accomplish this task; these mutated proteins interfere with the function of their normal cellular counterparts or with proteins that interact with them. This approach has been used extensively in the elucidation of signal-transduction cascades, such as those involving Ras-family proteins. Here I discuss the power and potential pitfalls of using dominant-inhibitory Ras proteins.Keywords
This publication has 26 references indexed in Scilit:
- Signal transduction from multiple Ras effectorsCurrent Opinion in Genetics & Development, 1997
- Distal Switch II Region of Ras2p Is Required for Interaction with Guanine Nucleotide Exchange FactorPublished by Elsevier ,1996
- Identification of residues critical for Ras(17N) growth-inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors.Molecular and Cellular Biology, 1994
- FUNCTION AND REGULATION OF RASAnnual Review of Biochemistry, 1993
- Studies on the structure and mechanism of H-ras p21Philosophical Transactions Of The Royal Society B-Biological Sciences, 1992
- Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP.Molecular and Cellular Biology, 1991
- Dominant inhibitory Ras mutants selectively inhibit the activity of either cellular or oncogenic Ras.Molecular and Cellular Biology, 1991
- Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1989
- Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP.Molecular and Cellular Biology, 1988
- Mutant ras-encoded proteins with altered nucleotide binding exert dominant biological effects.Proceedings of the National Academy of Sciences, 1986